Back to Tools
Biohacker Protocol

Peptide Injection Master Guide

Precision protocols for Subcutaneous (SubQ) and Intramuscular (IM) administration. Optimize absorption and minimize discomfort.

Subcutaneous (SubQ)

MOST COMMON

Injection into the fatty tissue layer just beneath the skin. Slow, sustained absorption. Ideal for most peptides (BPC-157, TB-500, GHK-Cu).

Best Sites
  • Abdomen (2 inches from navel)
  • Outer Thigh
  • Back of Arm
Needle Specs
Gauge29G - 31G
Length5/16" - 1/2"

Intramuscular (IM)

SPECIFIC USE

Injection deep into the muscle. Faster absorption. Used for larger volumes or specific compounds (e.g., Testosterone, some vitamins).

Best Sites
  • Deltoid (Shoulder)
  • Vastus Lateralis (Thigh)
  • Ventrogluteal (Hip)
Needle Specs
Gauge25G - 27G
Length1" - 1.5"

Injection Site Maps

ABDOMEN2" from Navel

The "Golden Zone"

The area 2 inches (5cm) away from the belly button on either side is ideal for SubQ injections.

  • Pinch the fat layer gently to separate it from muscle.
  • Rotate sites (left/right, upper/lower) to prevent scar tissue.
1

1. Prepare

Wash hands. Clean the vial top with an alcohol swab. Let it dry.

2

2. Draw

Inject air into the vial equal to your dose. Draw the peptide slowly to avoid bubbles.

3

3. Site Prep

Clean the skin site with a fresh alcohol swab. Let it dry completely (stings if wet).

4

4. Inject

Pinch fat (SubQ) or stretch skin (IM). Insert needle quickly. Push plunger slowly.

5

5. Finish

Withdraw needle. Do not rub the site. Dispose of needle in sharps container.

Safety First

Never reuse needles. If you hit a blood vessel (blood appears in syringe), withdraw and try a new site. Consult a healthcare professional before starting any injection protocol. This guide is for educational purposes only.

HomeResearchToolsVisualsLibraryContact